Table 1 Specific primer sequences
From: The anti-Alzheimer compounds from tempeh oil in LPS-induced neuronal Schwann cells
No | Gene | Annealing temperature (°C) | Amplicon (bp) | Sequence |
|---|---|---|---|---|
1 | TNFα | 55 | 319 | F: 5'GGCAGGTCTACTTTGGAGTCATTG'3 |
R: 5'ACATTCGAGGCTCCAGTGAATTCGG'3 | ||||
2 | cdk5 | 55 | 360 | F: 5’ ACTGTGTTCAAGGCTAAAAACC ‘3 |
R: 5’ CAATTTCAACTCCCCATTCC ‘3 | ||||
3 | Gsk3b | 58 | 368 | F: 5'CGAACTCCACCAGAGGCAAT'3 |
R: 5'TAAGTGCTGGTCTTCCCTGC'3 | ||||
4 | PSEN1 | 58 | 485 | F: 5' TAATGGCCGACCCCAGAGTA'3 |
R: 5'CAATCATCCCGACCACACCA'3 | ||||
5 | PSEN2 | 58 | 314 | F: 5' GTCTGATGAGCTGAGAGCCAG'3 |
R: 5'TGCTTCGCCCCATACTTGAG'3 | ||||
6 | B-actin | 58 | 439 | F: 5'TGGAATCCTGTGGCATCCATGAAAC'3 |
R: 5'TAAAACGCAGCTCAGTAACAGTCCG'3 |